Publications/IP

IP — AMNIONEURO – biologické krytí na bázi aminové membrány pro rekonstrukcní zákroky a biologické krytí


IP — Humane treatment containing mesenchymal stem cells and liquid medium


IP — Modulární lineární aktuátor pro aplikace v tkáňovém inženýrství


IP — Method for separation of sperm with undamaged intact heads from sperm with damaged heads and somatic cells


Ladislava Vymetalova1, Tereza Kucirkova1, Lucia Knopfova1, Veronika Pospisilova2,3, Tomas Kasko2,3, Hana Lejdarova4, Eva Makaturova5, Petr Kuglik5,6, Veronika Oralova7, Eva Matalova7, Petr Benes1, Zdenek Koristek2,8, Serhiy Forostyak2,3,8

Large‑Scale Automated Hollow‑Fiber Bioreactor Expansion of Umbilical Cord‑Derived Human Mesenchymal Stromal Cells for Neurological Disorders Neurochemical Research (2020) 45:204–214

Published: DEC 11 2019 ¦ PDF ¦ https://doi.org/10.1007/s11064-019-02925-y


1 – International Clinical Research Center, St. Anne’s University Hospital, Pekarska 53, 656 91 Brno, Czech Republic
2 – PrimeCell Bioscience Inc., Dr. Slabihoudka 6232/11, 708 00 Ostrava‑Poruba, Czech Republic
3 – National Tissue Centre Inc., Palachovo náměstí 726/2, 625 00 Brno, Czech Republic
4 – Department of Transfusion & Tissue Medicine, University Hospital Brno, Jihlavska 20, 625 00 Brno, Czech Republic
5 – Department of Medical Genetics, University Hospital Brno, 625 00 Brno, Czech Republic
6 – Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic
7 – Institute of Animal Physiology and Genetics, v.v.i, Czech Academy of Sciences, Veveri 97, 602 00 Brno, Czech Republic
8 – PrimeCell Advanced Therapy Inc., Palachovo nam. 726/2, 625 00 Brno, Czech Republic

IP — Contemplates methods for identifying candidate compounds or gene products


IP — AmniPur® – Method of preparation of amniotic membrane-based material (Czech Republic)


Dayanithi G1,2,3,4, Forostyak O5, Forostyak S6,7, Kayano T8,9,5, Ueta Y10, Verkhratsky A11,12

Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation. SCIENTIFIC REPORTS, Vol. 8. DOI: 10.1038/s41598-018-31361-1.

Published: AUG 30 2018


1 – Institut des Sciences Biologiques-Neurosciences, cognition, Centre Nationale de la Recherche Scientifique, 3 rue Michel-Ange, 75794, Paris cedex 16, France. gdaya@univ-montp2.fr.
2 – MMDN-Institut National de la Santé et de la Recherche Médicale-U1198, Université de Montpellier, 34095, Montpellier, France. gdaya@univ-montp2.fr.
3 – Ecole Pratique des Hautes Etudes, Sorbonne, Les Patios Saint-Jaques, 75014, Paris, France. gdaya@univ-montp2.fr.
4 – Department of Pharmacology and Toxicology, Faculty of Medicine, Charles University at Plzen, CZ-32300, Plzen, Czech Republic. gdaya@univ-montp2.fr.
5 – Department of Molecular Neurophysiology, Institute of Experimental Medicine, Czech Academy of Sciences, 14220, Prague, Czech Republic.
6 – Department of Neuroscience, 2nd faculty of Medicine, Charles University, V Uvalu 84, 15006, Prague, Czech Republic
7 – PrimeCell Therapeutics a.s. Palachovo Náměstí 2, 625 00, Brno, Czech Republic.
8 – MMDN-Institut National de la Santé et de la Recherche Médicale-U1198, Université de Montpellier, 34095, Montpellier, France
9 – Ecole Pratique des Hautes Etudes, Sorbonne, Les Patios Saint-Jaques, 75014, Paris, France.
10 – Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, 807-8555, Japan.
11 – Faculty of Biology, Medicine and Health, University of Manchester, M13 9PT, Manchester, UK. Alexej.Verkhratsky@manchester.ac.uk.
12 – Achucarro Centre for Neuroscience, IKERBASQUE, Basque Foundation for Science, 48011, Bilbao, Spain. Alexej.Verkhratsky@manchester.ac.uk.

Sykova E; Cejkova J; Cejka C; Forostyak S.

Collecting stem cells from bones or from line cells used for suppressing myocardial infarction and posttraumatic shock, and treating infections caused by treatment of any kind of disease. 2018. Patent Number: CZ201600644-A3; CZ307325-B6.

Published: 2018


Sykova E, Forostyak S, Rychmach P. Konradova S., Matejckova N.

A stem cell therapy of amyotrophic lateral sclerosis: from bench to bedside. Cytotherapy, Vol. 20, 5.

Published: MAY 2018


IP — AmniPur® – Method of preparation of amniotic membrane-based material and a material obtainable by said method


Štěpanovská J, Matějka R

Bioreaktor – továrna na umělé orgány, Pražská technika, 2/2018

Published: 2018 ¦ PDF ¦ https://media.cvut.cz/cs/publikace/20180416-prazska-technika-2-2018


Giulia E. Tyzack1,2, Claire E. Hall2, Christopher R. Sibley3, Tomasz Cymes1, Serhiy Forostyak4, Giulia Carlino2, Ione F. Meyer5, Giampietro Schiavo5,6, Su-Chun Zhang7, George M. Gibbons1, Jia Newcombe8, Rickie Patani2,9 and András Lakatos1,10

A neuroprotective astrocyte state is induced by neuronal signal EphB1 but fails in ALS models. Nature communications Vol: 8, 1164.

Published: OCT 27 2017


1 – John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, E.D. Adrian Building, Forvie Site, Robinson Way, Cambridge, CB2 0PY UK
2 – Department of Molecular Neuroscience, UCL Institute of Neurology, University College London, London, WC1N 3BG UK
3 – Division of Brain Sciences, Imperial College London, Burlington Danes Building Du Cane Road, London, W12 0NN UK
4 – Institute of Experimental Medicine ASCR and Charles University in Prague, Department of Neuroscience, Videnská 1083, Prague 4, 142 20 Czech Republic
5 – Sobell Department of Motor Neuroscience & Movement Disorders, UCL Institute of Neurology, University College London, London, WC1N 3BG UK
6 – UK Dementia Research Institute at UCL, UCL Institute of Neurology, University College London, London, WC1N 3BG UK
7 – Waisman Center, University of Wisconsin, 1500 Highland Avenue, Madison, WI 53705 USA
8 – Department of Neuroinflammation, UCL Institute of Neurology, University College London, London, WC1N 1PJ UK
9 – The Francis Crick Institute, 1 Midland Road, London, NW1 1AT UK
10 – Addenbrooke’s Hospital, Cambridge University Hospitals, Hills Road, Cambridge, CB2 0QQ UK Rickie Patani

Forostyak S1,2 and Sykova E.2,3

Neuroprotective Potential of Cell-Based Therapies in ALS:From BenchtoBedside. Frontiers in neuroscience. Vol: 11, 591.

Published: OCT 24 2017


1 – Centre of Reconstructive Neuroscience, Institute of Experimental Medicine (ASCR), Czech Academy of Sciences, Prague, Czechia
2 – Department of Neuroscience, 2nd Faculty of Medicine, Charles University, Prague, Czechia
3 – Institute of Neuroimmunology, Slovak Academy of Sciences, Bratislava, Slovakia

Sykova E1,2, Rychrnach P3, Drahoradova I3, Konradova S1,3, Ruzickova K1,3, Vorisek I1, Forostyak S1,2, Homola A1,4, Bojar M4

Transplantation of Mesenchymal Stromal Cells in Patients With Amyotrophic Lateral Sclerosis: Results of Phase I/IIa Clinical Trial. Cell transplantation Vol: 26, 4 (SI).

Published: 2017


1 – Department of Neuroscience, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic
2 – Department of Neuroscience, Charles University, 2nd Medical School, Prague, Czech Republic
3 – Bioinova Ltd., Prague, Czech Republic
4 – Department of Neurology, Charles University, 2nd Medical School, University Hospital at Motol, Prague, Czech Republic

Forostyak O, Butenko O, Anderova M, Forostyak S, Sykova E, Verkhratsky A, Dayanithi G.

Specific profiles of ion channels and ionotropic receptors define adipose- and bone marrow derived stromal cells. Stem cell research. Vol.: 16, 3.

Published: MAY 2016


Forostyak O, Forostyak S, Kortus S, Sykova E, Verkhratsky A, Dayanithi G.

Physiology of Ca2+ signalling in stem cells of different origins and differentiation stages. CELL CALCIUM. Vol: 59, 2-3 (Special Issue).

Published: 2016


Dmitry Tukmachev, MD1,2, Serhiy Forostyak, MD, PhD1,2, Zuzana Koci, MS1,2, Kristyna Zaviskova, MS1,2, Irena Vackova, PhD1, Karel Vyborny, MS1,2, Ioanna Sandvig, PhD3,4, Axel Sandvig, MD, PhD3,5, Christopher J. Medberry, PhD6, Stephen F. Badylak, DVM, PhD, MD6, Eva Sykova, MD, DSc1,2 and Sarka Kubinova, PharmD, PhD1

Injectable Extracellular Matrix Hydrogels as Scaffolds for Spinal Cord Injury Repair. Tissue engineering part A. Vol: 22, 3-4.

Published: 2016


1 – Institute of Experimental Medicine AS CR, Prague, Czech Republic.
2 – 2nd Medical Faculty, Charles University, Prague, Czech Republic.
3 – Department of Neuroscience, Norwegian University of Science and Technology, Trondheim, Norway.
4 – John Van Geest Centre for Brain Repair, School of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom.
5 – Division of Pharmacology and Clinical Neuroscience, Department of Neurosurgery, Umeå University, Umeå, Sweden.
6 – McGowan Institute for Regenerative Medicine, Pittsburgh, Pennsylvania

Sykova E, Forostyak S, Homola A, Konradova S, Ruzickova K, Syka M, Jendelova P.

Stem Cells for Treatment of Amyotrophic Lateral Sclerosis: Preclinical and Clinical Study. Cell transplantation. Vol: 25, 4.

Published: 2016


Kaláb M, Karkoška J, Kamínek M, Šantavý P.;
Transplantation of allogeneic bone graft in the therapy of massive post-sternotomy defects – 6 years of experience with the method; Rozhl Chir. Fall 2016;95(11):399-406. Czech.
Published: 2016 ¦ PDF ¦ https://www.medvik.cz/bmc/link.do?id=bmc17000120


Martin Kaláb1, Jan Karkoška2, Milan Kamínek3, Eva Matějková2, Zuzana Slaměníková1, Aleš Klváček1 and Petr Šantavý1

Reconstruction of massive post-sternotomy defects with allogeneic bone graft: four-year results and experience using the method. Interact CardioVasc Thorac Surg 2016;22:305–13.

Published: 2015 Nov 29 ¦ PDF ¦ https://doi.org/10.1093/icvts/ivv322


1 – Department of Cardiosurgery, University Hospital Olomouc and Faculty of Medicine, Palacky University Olomouc, Olomouc, Czech Republic
2 – National Cell and Tissue Centre, Brno, Czech Republic
3 – Department of Nuclear Medicine, University Hospital Olomouc and Faculty of Medicine, Palacky University Olomouc, Olomouc, Czech Republic

Kubinova S, Horak D, Hejcl A, Plichta Z, Kotek J, Proks V, Forostyak S, Sykova E.

SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair. Journal of tissue engineering and regenerative medicine. Vol.: 9, 11.

Published: NOV 2015


Kubinova S, Tukmachev D, Vyborny K, Koci Z, Zaviskova K, Vackova I, Forostyak S, Sykova E.

Extracellular Matrix Hydrogels as Scaffolds for Spinal Cord Injury Repair. Tissue engineering part A. Vol: 21, Supp.1.

Published: SEP 1 2015


NARDONE, Giorgia, Jorge Oliver de la CRUZ, Martin PEŠL, Stanislava KOŠKOVÁ a Giancarlo FORTE

YAP mechanosensor as a crucial determinant in the differentiation of adipose tissue-derived mesenchymal stem cells, ISSCR meeting, Stokholm on June 24-27/2015

Published: 2015 June 24-27 ¦ PDF ¦ https://is.muni.cz/publication/1301857/cs


Raha-Chowdhury R1, Animesh Alexander Raha1,2, Forostyak S3, Zhao JW1, Stott SRW1, Bomford A.2

Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neuroscience. Vol: 16, 24.

Published: APR 21 2015.


1 – John Van Geest Centre for Brain Repair, Department of Clinical Neuroscience, University of Cambridge, Cambridge, UK
2 – Institute of Liver Studies, King’s College Hospital, London, UK
3 – Department of Neuroscience Institute of Experimental Medicine Academy of Sciences of the Czech Republic, Prague, Czech Republic

Martin Kaláb1, Jan Karkoška2, Milan Kamínek3 and Petr Šantavý1

Successful three-year outcome in a patient with allogenous sternal bone graft in the treatment of massive post-sternotomy defects; International Journal of Surgery Case Reports 7 (2015) 6–9,

Published: 2014 Dec 24 ¦ PDF ¦ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336405/


1 – Department of Cardiosurgery, University Hospital Olomouc and Faculty of Medicine, Palacky University Olomouc, I. P. Pavlova 6, Olomouc 775 20, Czech Republic
2 – National Cell and Tissue Centre, Palachovo náměstí 726/2, Brno, Czech Republic
3 – Department of Nuclear Medicine, University Hospital Olomouc and Faculty of Medicine, Palacky University Olomouc, I. P. Pavlova 6, Olomouc 775 20, Czech Republic

Serhiy Forostyak1,2, Ales Homola1,2, Karolina Turnovcova1,2, Pavel Svitil1,2, Pavla Jendelova1,2 and Eva Sykova1,2

Intrathecal Delivery of Mesenchymal Stromal Cells Protects the Structure of Altered Perineuronal Nets in SOD1 Rats and Amends the Course of ALS. STEM CELLS. Vol: 32, 12.

Published: DEC 2014


1 – Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic
2 – Department of Neuroscience, 2nd Medical Faculty, Charles University, Prague, Czech Republic

Kubinova S, Tukmachev D, Forostyak S, Hejcl A, Horak D, Plichta Z, Proks V, Medberry C, Sykova E.

Comparison of synthetic versus biologic hydrogel scaffolds in spinal cord injury treatment. Journal of tissue engineering and regenerative medicine. Vol: 8, Special Issue.

Published: JUN 2014


Miriama Krutá1,2, Monika Šeneklová1, Jan Raška1, Anton Salykin1, Lenka Zerzánková1, Martin Pešl1,2, Eva Bártová3, Michal Franek3, Aneta Baumeisterová1, Stanislava Košková4, Kai J. Neelsen5, Aleš Hampl6, Petr Dvořák1,2 and Vladimír Rotrekl1,2

Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts. Stem Cells and Development, Mary Ann Liebert, Inc., 2014. ISSN 1547-3287.

Published: 2014 May 17 ¦ https://www.liebertpub.com/doi/10.1089/scd.2013.0611


1 – Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
2 – International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, Czech Republic.
3 – Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
4 – National Tissue Centre, Inc., Brno, Czech Republic.
5 – Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark.
6 – Department of Histology and Embryology, Masaryk University, Brno, Czech Republic.

Forostyak S, Jendelova P, Sykova E.

The role of mesenchymal stromal cells in spinal cord injury, regenerative medicine and possible clinical applications. BIOCHIMIE. Vol.: 95, 12.

Published: DEC 2013.


Sykova Eva1,2, Forostyak Serhiy1

Stem cells in regenerative medicine. Laser therapy. Vol: 22, 2.

Published: 2013.


1 – Department of Neuroscience, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Videnska 1083, Prague, 14220, Czech Republic
2 – Department of Neuroscience, 2nd Faculty of Medicine, Charles University, V Uvalu 84, Prague, 15006, Czech Republic

Kubinova S, Hejcl A, Forostyak S, Baranovicova L, Horak D, Plichta Z, Proks V, Sykova E.

Biomodified hydrogel scaffolds for neural differentiation and the treatment of spinal cord injury. Journal of tissue engineering and regenerative medicine. Vol: 6, Special Issue, Supplement 1.

Published: SEP 2012.


Kalab M1, Kubesova B2 and Lonsky V1

Use of allogenous bone graft and osteosynthetic stabilization in treatment of massive post-sternotomy defects, European Journal of Cardio-Thoracic Surgery 41 (2012) e182–e184;

Published: 2012 Apr 18 ¦ PDF ¦ doi:10.1093/ejcts/ezs077


1 – Department of Cardiac Surgery, University Hospital and Faculty of Medicine, Palacky University, Czech Republic
2 – National Tissue Centre, Brno, Czech Republic

Forostyak S, Homola A, Jendelova P, Sykova E.

The intrathecal application of human bone marrow mesenchymal stromal cells improves motor activity, prolongs survival and preserves perineuronal nets in symptomatic ALS rats.

Published: 2012.


Vanecek V, Zablotskii V, Forostyak S, Ruzicka J, Herynek V, Babic M, Jendelova P, Kubinova S, Dejneka A, Sykova E.

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury. International journal of nanomedicine. Vol: 7.

Published: 2012.


Arboleda D, Forostyak S, Jendelova P, Marekova D, Amemori T, Pivonkova H, Masinova K, Sykova E.

Transplantation of Predifferentiated Adipose-Derived Stromal Cells for the Treatment of Spinal Cord Injury. Cellular and molecular neurobiology. Vol: 31, 7.

Published: OCT 2011.


Forostyak S, Jendelova P, Kapcalova M, Arboleda D, Sykova E.

Mesenchymal stromal cells prolong the lifespan in a rat model of amyotrophic lateral sclerosis. Cytotherapy. Vol: 13, 9.

Published: OCT 2011.


Dayanithi G, Forostyak O, Forostyak S, Arboleda D, Viero C, Strunin D, Folkova D; Sykova E, Shibuya I, Ueta Y, Toescu EC, Verkhratsaky.

Neuron-glia interactions in peripheral vasopressin and oxytocin systems unveiled in transgenic rats. GLIA. Vol: 59, Supplement: 1.

Published: OCT 2011.


Dayanithi G, Forostyak O, Forostyak S, Arboleda D, Ueta Y, Sykova E.

Transgenic rat models to visualize fluorescent vasopressin and oxytocin in the dorsal root ganglia and glial cells. Vol: 41.

Published: 2011.


Forostyak S, Jendelova P, Kapcalova M, Arboleda D, Sykova E.

Mesenchymal stem cells prolong lifespan in a rat model of amyotrophic lateral sclerosis. Vol: 41.

Published: 2011


Carulli D, Pizzorusso T, Kwok JCF, Putignano E, Poli A, Forostyak S, Andrews MR, Deepa SS, Glant TT, Fawcett JW. (Fawcett,
James W.

Animals lacking link protein have attenuated perineuronal nets and persistent plasticity. BRAIN. Vol: 133, 8.

Published: AUG 2010


Arboleda D, Forostyak S, Sykova E, Jendelova P.

The use of adipose tissue-derived mesenchymal stem cells in spinal cord injury repair. Vol: 39.

Published: 2009.